The herbicide Atrazine was recently featured in a research study at University of California at Berkeley, and the reporting story–Pesticide atrazine can turn male frogs into females–stated that 75% of frogs exposed to Atrazine were chemically emasculated, and 1 in 10 was turned into a female!
Atrazine was banned in the European Union but continues to be one of the most widely used herbicide in conservation tillage systems, which are designed to prevent soil erosion. Because of widespread and long-term usage, Atrazine (along with other triazine herbicide metabolites) can be found in the environment and groundwater at low levels. Monitoring of Atrazine metabolites desethyl-atrazine and desisopropylazine is important for environmental protection and food safety control because its consumption at levels lower than those mandated by various governments has been linked to birth defects, and endocrine and possible carcinogenic effects in humans.
Here, we present an extraction solution using our Accelerated Solvent Extraction (ASE) technique and then determination of triazine pesticides using a reversed-phase liquid chromatography (LC) column!
Application Note 359: Extraction of Contaminants, Pollutants, and Poisons from Animal Tissue Using Accelerated Solvent Extraction (ASE) (downloadable PDF) describes the fast and excellent extraction of atrazine from beef kidney samples spiked with different concentrations of Atrazine. (Procedures on extracting dioxins/furans, Polybrominated Flame Retardants (PBDE), PCBs, PAHs, and organotin are also included.) The ASE technique is approved under EPA Method 3545A (downloadable PDF) for extraction of these contaminants and pollutants from soils and sediments.
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Here is a chromatogram showing the separation of Atrazine and other herbicides on our Thermo Scientific Acclaim 120 C18 column and an acetonitrile/water gradient, followed by a sensitive determination using LC/MS.
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