hesperidin in orange peelHesperidin is a antioxidant bioflavonoid present in the peel of citrus fruits and is used in natural health products. It is reported to work as an anti-inflammatory, antihistaminic, and antiviral agent. Hesperidin is also being used as a source of vitamin P in experimental medicine. It cannot be manufactured by the human body and must be supplied in the diet.

Here, is a fast application significantly improving the Pharmacopoeia of the People’s Republic of China (PPRC) 2010 method. This application was developed at our Shanghai Corporate Applications lab on the request of a customer based in China.

The PPRC 2010 recommends the extraction of hesperidin from citrus fruits with a Soxhlet extraction method using ligarine and methanol solvents, which requires ≥5 h and >200 mL of solvents for each sample followed by its determination using a reversed-phase high-performance liquid chromatography (HPLC) method.

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Application Brief 142, Rapid Determination of Hesperidin in Orange Peel Using Accelerated Solvent Extraction and UHPLC, (downloadable PDF) describes the use of Accelerated Solvent Extraction (ASE) to extract hesperidin from orange and lemon peels in 35 min. The extraction is followed by separation by ultra high-pressure liquid chromatography (UHPLC) on a short, rapid-separation LC (RSLC) column (Thermo Scientific Acclaim 2.2 µm 120 C18 column). These ultrafast, high-efficiency RSLC columns can accelerate separations up to 15-fold compared to conventional LC columns. In this application, the separation required less than 5 min.

Do check out some of our other applications that determine strong antioxidants by UHPLC:

Do comment below if you are interested in the analysis of other antioxidants. I can pass the word along to our application chemists!