An earlier series of blog posts focused on the HPLC analysis of Chinese traditional medicines (Post 1 and Post II) and generated a lot of interest in our readers. This blog post focuses on three herbal medicines that are extremely popular in many Western countries and our applications demonstrate excellent results in the detection of analytes.

Faster Analysis of Milk Thistle Extracts by UHPLC-UV-CAD

Milk-thistle-has-long-been-used-as-an-herbal-medicine-and-dietary-supplement-for-promoting-digestive-health-in-the-West.-(This-plant-is-native-to-the-Mediterranean-part-of-Europe-and-was-first-described-byDioscorides,-a-Greek-physician-in-the-Roman-army,-in-<a-href=”http://penelope.uchicago.edu/~grout/encyclopaedia_romana/aconite/materiamedica.html”-title=”De-Materia-Medica”-target=”_blank”>De-Materia-Medica.)

Here-we-present-how-our-HPLC-application-for-the-analysis-of-botanical-supplement-milk-thistle,-based-on-the-established-Institute-for-Nutritional-Advancement-(INA)-<a-href=”http://www.nsf.com/business/ina/milkthistle.asp?program=INA”-title=”Method-115.00-for-silymarins-in-milk-thistle-extracts”-target=”_blank”>Method-115.00-for-silymarins-in-milk-thistle-extracts,-was-easilyconverted-to-<a-href=”http://www.dionex.com/en-us/products/liquid-chromatography/lc-systems/lp-87043.html”-title=”UHPLC”-target=”_blank”>UHPLC-conditions-with-our-<a-href=”http://www.dionex.com/en-us/products/liquid-chromatography/lc-solutions/rapid-separation-lc/lp-80946.html”-title=”Method-Speed-Up-Calculator”-target=”_blank”>Method-Speed-Up-Calculator-(scroll-to-below-the-fold-to-click-the-link-to-the-tool).-Using-the-Thermo-Scientific-Dionex-UltiMate-3000-HPLC-system,-the-Thermo-Scientific-ESA-Corona-Charged-Aerosol-Detector-(<a-href=”http://www.dionex.com/en-us/products/liquid-chromatography/lc-modules/detectors/charged-aerosol/corona-ultra/lp-85220.html”-title=”CAD”-target=”_blank”>CAD),-and-our-<a-href=”http://www.dionex.com/en-us/products/columns/lc/reversed-phase/acclaim-rapid-separation-lc/lp-71766.html”-title=”rapid-separation-LC-column”-target=”_blank”>rapid-separation-LC-column-(Thermo-Scientific-Acclaim-RSLC-120-C18-2.2-┬Ám-column),the-improved-method-is-5X-faster-while-enhancing-resolution.-Our-<a-href=”http://www.dionex.com/en-us/products/liquid-chromatography/lc-modules/detectors/lp-72497.html”-title=”UV”-target=”_blank”>UV-and-<a-href=”http://www.dionex.com/en-us/products/liquid-chromatography/lc-modules/detectors/charged-aerosol/lp-85214.html”-title=”CAD-detectors–“-target=”_blank”>CAD-detectors-complement-one-another,-with-the-CAD-providing-nearly-universal-detection-of-nonvolatile-analytes,-similar-inter-analyte-response-independent-of-chemical-structure,-and-higher-sensitivity,-wider-dynamic-range,-and-more-tractable-calibration-behavior-over-ELSD.-View-the-<a-href=”http://dlibrary.dionex.com/Public/View.aspx?ApplicationID=266″-title=”results-in-our-D-Library”-target=”_blank”>results-in-our-D-Library.

UV-Absorption-Detection-of-Kavalactones-in-Kava-Kava-Extracts

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<img-id=”img-1332275873585″-src=”http://chromblog.thermoscientific.com/Portals/49739/images/Kava-Root.jpg” border=”0″ alt=”UV absorption detection of kavalactones” width=”157″ height=”122″ class=”alignRight” style=”float: right;”>Kavalactones are the active ingredient in the roots of the Kava (or Kava Kava), a shrub commonly found on many of the Pacific Islands. It is considered to have muscle relaxant, anti-convulsive, and anesthetic properties. Kava preparations are sold in the U.S. as capsules or teas.

The application determines kavalactones in Kava Kava extracts using UV absorption detection at 246 nm.

UV Absorption Detection of Phenolic Compounds in Echinacea Extracts

phenolic compounds in echinacea extractsHerbalists consider echinacea one of the best blood purifiers and also an effective antibiotic and it remains one of the top selling herbal remedies in the United States. Constituents of echinacea include polysaccharides, polyacetylenes, sesquiterpenes, polyphenols, and more.

Our application determines phenolic compounds in echinacea extracts using UV absorbance detection at 330 nm and the chromatogram shows separation and detection of several polyphenolic compounds in echinacea extract.

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