After the blog post on the EFSA re-evaluating the safety of aspartame and the one on Mogroside V from luo han kuo (Siraitia grosvenorii) fruit extract, the next blog post in this series on the analysis of artificial and natural sweeteners is on the analysis of stevia, a natural sweetener. Four years ago, rebaudioside A (a steviol glycoside purified from the stevia plant) received Generally Recognized as Safe (GRAS) status from the US FDA, and since then it has because increasingly popular as a tabletop sweetener and is included in many food and beverage products as a sugar substitute. Incidentally, stevia has been used as a sweetener in food products in Japan since 1970.
Here we present a new application, Application Note 293, Determination by HPLC with Charged Aerosol and UV Detections Using the Acclaim Trinity P1 Column, (downloadable PDF) which improves on a previously published study: Application Note 241
Determination of Steviol Glycosides by HPLC with UV and ELS Detections (downloadable PDF).
This new method uses our unique, trimode liquid chromatography column (Thermo Scientific Acclaim Trinity P1 column) in the hydrophilic interaction liquid chromatography (HILIC) mode to separate six steviol glycosides and Mogroside V with charged aerosol detection (CAD) (Thermo Scientific Dionex ESA Charged Aerosol Detector). The advantage of CAD is that it is more sensitive and has a greater linear range than ELSD, and is not hampered with the structural bias of an ELSD.
And, last but not least, here is a great poster, Sensitive Analysis of Commonly Used Artificial and Natural Sweeteners Including Stevia and Their Impurities and Degradation Products,(downloadable PDF) demonstrating the use of HPLC-CAD to measure sugars in honey, various components in Splenda®, active ingredients and impurities in Equal®, and the analyte profiles of commercially available Stevia products. The poster presents a global method capable of simultaneously measuring nine artificial sweeteners! For those only interested in the analysis of Stevia extracts (rebaudioside A and stevioside), note that these are analyzed using HPLC with our CAD detector and UV.
Like what you are learning?
How Charged Aerosol Detection Works
Check out this 3 min 22 sec video on the technique of CAD.
Let us know of your analytical challenges in the Comments box below. Also, do let us know if the video was helpful. We look forward to hearing from you!