Continuing our series on applications for the analysis of tobacco with this second post on this topic. The first blog post presented the fast HPLC analysis of tobacco-specific nitrosamines and also mentioned that as a part of implementing Act H.R. 1256, the US FDA’s Center for Tobacco Products (CTP) released an initial list of 93 Harmful and Potentially Harmful Constituents (HPHCs) chemicals or chemical compounds in tobacco and tobacco products; and, recently, the CTP released a shortened list of 20 chemicals that must be reported on by tobacco companies by the end of 2012.
Here, we discuss Environmental Tobacco Smoke (ETS) which is defined as sidestream and mainstream smoke exhaled by smokers. By now, the relation between ETS and numerous disease states and mortality is well documented: in 1986, the U.S. Surgeon General and the Expert Committee on Passive Smoking, National Academy of Sciences’ National Research Council (NAS/NRC) published studies on ETS exposure and adverse health effects in nonsmokers. The US EPA page on Health Effects of Exposure to Secondhand Smoke also provides details on adverse health effects and the science behind this issue.
Typically, nicotine in air, surface, and dust samples has been used as a marker for ETS exposure assessment for decades but methods to quantify nicotine using reversed-phase HPLC methods suffer from significant peak tailing that negatively effects quantification at low concentrations.
We present for the first time a validated method for nicotine analysis from ambient air, surface, and dust samples! The method described in this poster, titled, A Validated Analytical Method for Environmental Nicotine Exposure by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry, (downloadable PDF) describes the extraction of samples by solid phase extraction (SPE) and then quantification by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry (HILIC–MS/MS) with electrospray ionization (ESI). The ruggedness of the method was verified by analyzing hundreds of environmental nicotine samples from dosimeters, surface wipes, and dust.
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Note that this poster also briefly describes using the same method for nictotine bioanalysis in urine and reports that excellent method performance was observed for quantification of nicotine in urine samples!
Next in this series, we will cover an article on the analysis of nitrosamines in water using automated solid-phase extraction & GC-MS!
Let us know if this application note was helpful to you in your work! We look forward to hearing from you.