In this blog post, we cover the analysis of carbohydrates, which are difficult to analyze because they have similar physical and chemical characteristics, and do not have a suitable chromophore for UV detection.
The various methods were developed using our HPLC system (Thermo Scientific Dionex UltiMate 3000 HPLC system) with Pulsed Amperometric Electrochemical (PAD) detection (see link to Technical Note at end of blog post below) and are described in the poster note, Carbohydrate Analysis using HPLC with PAD, FLD, Charged Aerosol Detection, and MS Detectors (downloadable PDF).
The following simple, rapid and accurate methods are included:
- Carbohydrate analysis in a pharma application by HPLC-EC PAD: A simple PAD method was developed using our electrochemical detector (Thermo Scientific Dionex Coulochem III ED) in pulse mode. The application tested impurities in 2-deoxy-2-[18F]fluoro-D-glucose (FDG), the most widely used radiopharmaceutical for positron emission tomography (PET).
- Analysis of glycan derivatives with Fluorescence Detection: In this application, the derivatizing agent 2AA was used and the method showed excellent sensitivity and selectivity.
- Carbohydrate analysis in fruit juices with Charged Aerosol Detection (CAD): The application measured carbohydrates in a variety of fruit juice samples and was performed using a polymeric amino column in HILIC mode.
- Glycoprotein analysis by parallel CAD and Mass Spectrometry: The application was for the identification and quantification of glycans from fetuin.
Also, I found this technical note, Technical Note 21, Optimal Settings for Pulsed Amperometric Detection of Carbohydrates Using the Dionex ED40 Electrochemical Detector, (downloadable PDF) to assist you in using the ED.
Do let us know in the Comments box below if you are looking for more HPLC applications for carbohydrate analysis: our application experts will be pleased to consider your request.