Here, we present an alternative method to the commonly used HPLC with fluorescence detection method for those who need to determine carbofuran and carbaryl in environmental waters. (N-methylcarbamates are widely used agricultural pesticides and are considered to be toxic to humans.)
The commonly used method for the sensitive determination of carbamates, as per US EPA Method 531.2 and EPA Method 8318, is reversed-phase high-performance liquid chromatography (RP-HPLC) with fluorescence detection following postcolumn derivatization. But, this method besides being time consuming, requires large volumes of organic solvents, and is deficient in terms of process control.
Here we present a much more efficient and faster method described in Application Update 186, Rapid HPLC Determination of Carbofuran and Carbaryl in Tap and Environmental Waters Using On-Line SPE (downloadable PDF). The note describes using our HPLC system (Thermo Scientific Dionex UltiMate 3000 x2 Dual LC system), on-line Solid-Phase Extraction (SPE) of 2.5 mL of sample using our SPE cartridge (Thermo Scientific SolEx HRP cartridge), followed by a 5 min separation on a reversed-phase LC column (Thermo Scientific Acclaim 120 C18 column). The method allows determination of the titled carbamates at concentrations that meet regulatory requirements.
Also, if you are need to determine or screen for the full set of EPA or EU regulated carbamates in environmental waters, check out these application notes:
- Application 96, Determination of N-Methylcarbamates by Reversed-Phase HPLC,(downloadable PDF), describes the postcolumn derivatization with fluorescence detection after a reversed-phase HPLC separation is consistent with U.S. EPA Method 531.2 (link to method above). This method delivers highly sensitive determinations of carbamate compounds.
- Application Update 177, Faster and More Sensitive Determination of N-Methylcarbamates in Drinking Water by HPLC, (downloadable PDF), describes a faster and more sensitive method for the determination of the carbamates specified in EPA Method 531.2 and also meets the requirements of regulations of the European Union (EU) for drinking water provide a general rule for pesticides (98/83/EC).
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