In this third blog post on the use of chromatography in air analysis (link to previous blog posts), I am pleased to tell you about a recently developed efficient HPLC method for the determination of carbonyl compounds in air samples from vehicle passenger cabins.
As mentioned in the first post in this series, carbonyl compounds known to cause adverse effects on human health and are hence considered to be hazardous compounds and regulated by the following regulatory methods: California Air Resources Board Method No. 1004, International Organization for Standardization 16000-3:2011, U.S. Environmental Protection Agency (EPA) Compendium Method TO-11A, and the Chinese HJ/T 400-2007. (Links to the regulations can be found in the first blog post in this series.)
Exposure to carbonyl compounds in automotives occurs in the following ways:
- During the manufacture of carbonyl compounds as these compounds are widely used in large quantities in automotive manufacturing.
- Release of carbonyl compounds into cabin compartments of new vehicles as people in modern society spend more time in vehicles due to lengthy commutes, long-distance travel, and frequent traffic jams.
As regulatory bodies have limited the amounts of the carbonyl compounds that can be in the cabin compartment of a new automobile, it is, therefore, important to establish effective methods for the determination of thesecompounds found in the airinside vehicle cabins.
Application Note 1056, Determination of Carbonyl Compounds in Vehicle Cabin Compartments, (downloadable PDF) was developed by our Shanghai Applications Lab and the preparation for vehicle passenger cabin air samples (including pretreatment, sampling, and elution) was performed by a third-party inspection institution located in Shanghai, China, and was based on the standard methods enacted by the Chinese government.
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For the method development, chemists used two of our rapid-separation HPLC systems (Thermo Scientific Dionex UltiMate 3000 RSLC system) equipped with UV detectors (Thermo Scientific Dionex DAD-3000RS Diode Array Detector) plus one of our reversed-phase HPLC columns (Thermo Scientific Acclaim 120 C18 Analytical Column) and one of our silica HPLC columns (Thermo Scientific Syncronis C18 HPLC Column).
The method demonstrates that all 13 carbonyl compounds specified in the above mentioned regulations are derivatized with 2,4-dinitrophenylhydrazine and well separated in less than 10 minutes.
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