HPLC Troubleshooting ToolsOne of our HPLC experts,Holger Franz, from our production and development site in Germering, Germany, sent this really helpful post listing all the HPLC troubleshooting tools that we have developed in the last year.


Isn’t it always that HPLC issues arise when you are already late? I can recall evenings when I desperately tried to dig to the root cause of an issue and finally gave up. Next morning, the issue was so obvious; I could fix it quickly and get the job done. Troubleshooting an intermittent HPLC problem challenges your creativity. Sometimes you need to step back and look at the issue from a different perspective. But even the toughest problems eventually get solved with a combination of persistence and technical acumen.

To help with this, I can recommend a number of tools described below.

HPLC Troubleshooting Poster

Hang this complementary (and very popular) HPLC Troubleshooting Poster (link to order the poster) in your lab or office for quick and handy reference and fix the problem fast. This attractive poster includes solutions to a multitude of problems with your baseline, detector, column, inlet filter, and results. This guide is a special project supplement by LC/GC North America sponsored by Thermo Scientific.

HPLC Troubleshooting Mobile Application—The World of HPLC Troubleshooting at Your Fingertips

When you need help with HPLC application or system troubleshooting, you need it now. This interactive application contains a large number of solutions to various troubleshooting topics. All solutions are just a few clicks away and can easily be shared with a colleague.

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The HPLC Troubleshooting Application (link to free download of application) is available for iPhone, Android, and Windows phones. You can also scan this quick response (QR) code:

HPLC Troubleshooting App QR Code 150x150

Case Study: HPLC Troubleshooting Application Saves Time and Frustration

This last resource is a case study on solving negative peaks during HPLC method development using the mobile application (link to full PDF). The case study walks you through the steps of identifying the root cause of the issue and explains the technical background. Once you have the solution, you can then share it with your team using your contacts list!

If you’ve been working with HPLC for any length of time, you certainly have some troubleshooting war stories of your own. What’s the toughest technical problem you ever solved, and what was the key that unlocked your solution? Tell us in the Comments box below. We look forward to hearing from you!