anthocyanin analysisBetween 2006 and 2008, almost 320 drink products containing pomegranate juice or flavor were launched into the marketplace. The reason for this huge popularity is that pomegranate juice has been reported to contain three times more antioxidant activity than green tea and higher total polyphenol concentrations compared to common fruit juices, such as orange, grapefruit, grape, cranberry, pineapple, and apple.

This, of course, has led to a huge demand for pomegranates but given that the demand is outstripping the supply, there has been widespread adulteration of pomegranate juice in such drink products. Typical filler ingredients include cane sugar, corn syrup sweeteners, and lower-quality juices (grape, apple, or blackberry- containing sorbitol, malic acid, and sucrose.

An International Multidimensional Authenticity Specification (IMAS) Algorithm for Detection of Commercial Pomegranate Juice Adulteration has been developed as an authentication criterion which defines the anthocyanin profile in pomegranate juice consisting of a constant group of six anthocyanins irrespective of the origin of the juice.

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The Chromatography applications lab at Thermo Fisher Scientific has developed a sensitive and accurate HPLC method to separate and quantify anthocyanins in different fruit juices with a simple dilution of the sample. Application Note 264 describes a high-resolution, silica-based, Thermo Scientific Acclaim RSLC C18 column and absorbance detection at a visible wavelength of 540 nm to separate and detect anthocyanins in < 5 min.

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