chromatography analysis of vitamins in nutritional supplementsThis is the last of three blog posts on the analytical solutions for the determination of vitamins in nutritional supplements and functional waters to ensure product quality and regulatory compliance (US FDA dietary supplement regulatory information) as well as to monitor vitamin intake. In case you have not as yet read the first two posts, I do encourage you to check them out as they include links to downloadable application notes and a great poster. As mentioned in the previous posts, the market for both areas is rapidly growing globally. For example, according this story, the nutritional supplement market in India is expected to double by 2013. (The story also provides an overview of the regulatory situation in India for this nutritional supplements.)

Here are two more application notes plus a link to an LCGC article on this topic.

Simultaneous Analysis of Water-Soluble Vitamins in Vitamin-Enriched Beverages and Multivitamin Dietary Supplements

Based on their solubility, vitamins are divided into two categories: watersoluble vitamins (WSV) and fat-soluble vitamins (FSV). WSVs include vitamin C (ascorbic acid), B1 (thiamine), B2 (riboflavin), B3 (niacin, niacinamide), B5 (pentothenic acid), B6 (pyrodoxine), B7 (biotin), B9 (folic acid), and B12 (cyanocobalamine). Application Note 294, Simultaneous Analysis of Water-Soluble Vitamins in Vitamin-Enriched Beverages and Multivitamin Dietary Supplements by UHPLC-MS/MS, (downloadable PDF) presents a high-throughput method for simultaneous determination for all 10 WSVs using ultrahigh-performance LC and tandem mass spectrometry (UHPLC-MS/MS).

Separation of Water- and Fat-Soluble Vitamins in a Single Analysis

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Application Note 252, HPLC Assay of Water-Soluble Vitamins, Fat-Soluble Vitamins, and a Preservative in Dry Syrup Multivitamin Formulation, (downloadable PDF) describes the use of Thermo Scientific Acclaim PA2 Reversed-Phase LC column to successfully analyze a sample from 100% aqueous to 100% organic solvent, thereby allowing water- and fat-soluble vitamins to be separated in a single analysis. The Thermo Scientific Dionex UltiMate 3000 system and Thermo Scientific Chromeleon chromatography data system software facilitate this analysis by allowing multiple injections during the same run.

Separation of Vitamin K Isomers

And, last but not least, here is an article, Separation of Vitamin K Isomers, (downloadable PDF) that appeared in the LCGC Application Notebook demonstrating enhanced selectivity without excessive retention times for these Vitamin K isomers.

In case you are interesting in testing for other compounds in nutritional supplements, visit our website for analytical applications for a range of dietary and herbal supplements.

Also, don’t hesitate to comment below if you would like more details or found this information useful. We look forward to hearing from you!