biotechnology pharmaceutical analysisAre you working in the field of regulatory and analytical sciences for biotechnology pharmaceuticals? The reason for my asking is because I am very pleased to share with you seven posters (six using the HPLC technique and one using the Ion Chromatography technique), two seminar presentations, and plus other useful literature that was featured by our experts at the recent 2013 Well Characterized Biotechnology Pharmaceuticals (WCBP) Conference. We thought they would be useful to those of you who could not attend the conference.

Once you fill out a quick registration form (link to form), you can download the posters, presentations, and literature listed in this blog post!

Technical Posters

Our posters (title of this section is a link to the registration form) covered a wide range of solutions including novel column technology for glycan analysis and optimal separation and detection conditions for monoclonal antibody (MAb) analysis.

  • IgG Oligosaccharide Analysis by HPAE-PAD: Presents the development of a method to analyze and identify N-linked oligosaccharides from polyclonal IgG and MAbs.
  • A Novel pH Gradient Separation Platform for Monoclonal Antibody (MAb) Charge-Variant Analysis: Presents a method for generating a linear pH gradient for MAb charge variant separation.
  • Monitoring Protein PEGylation with Ion Exchange Chromatography: Presents the development of a method to characterize a mixture of proteins after branched polyethylene glycol (PEG)ylation using cation-exchange chromatography with mass spectrometry (MS) to evaluate the degree of PEGylation of model proteins and to separate mixed isomers present under different PEGylation conditions.
  • Novel Glycan Column Technology for the LC-MS Analysis of Labeled and Native N-Glycans Released from Proteins and Antibodies: Presents the development of novel HPLC and UHPLC columns for high-resolution separation and structural characterization of native and fluorescently labeled N-glycans released from various proteins, including antibodies. The UHPLC method uses fluorescence detection for the chromatographic analysis of labeled N-glycans and the HPLC method uses LC-MS and LC-MS/MS analysis for structural characterization of both labeled and native N-glycans from proteins by MS detection.
  • Performance Improvements for High Resolution Anion-Exchange Oligonucleotide Separations Using Small Particle Substrates: Presents the development of oligonucleotide (ON) separation columns for the Thermo Scientific DNAPac PA200 product line: a 150 mm-length size which provides increased throughput and resolution comparable to the DNAPac PA200 4 × 250 mm column; a 250 mm-length size which provides improved resolution and throughput comparable to the DNAPac PA200 4 × 250 mm column; and a 50 mm-length size which may be used as a guard or fast analytical column. These columns employ 4 μm particles and tolerate pressures up to 750 bar (11,000 psi).
  • Protein A Affinity Column for Monoclonal Antibody (MAb) Titer Analysis: Demonstrates the capability of a prototype Protein A column for titer analysis of MAbs using an HPLC hybrid system.

 

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Technical Presentations

The two technical presentations (title of this section is a link to the registration form) came from experts in our European offices.

  • New Developments in Chromatography for Biologics Development, Identification and QC: Presented by Wim Decrop, Research Scientist Chromatography, from our Amsterdam, The Netherlands, office, the presentation focuses on our new UHPLC system (Thermo Scientific Dionex UltiMate 3000 BioRS) designed for demanding applications in biopharmaceutical analysis.
  • New Developments in Mass Spectrometry for Improved Performance, Speed and Data Confidence in Biopharmaceutical Analytics: Presented by Dr. Kai Scheffler, Biopharma Market Development Manager Europe, the presentation covers intact antibody characterization using the new generation Orbitrap mass analyzer.

 

Literature

The download page (title of this section is a link to the registration form) includes the following two brochures and three application notes that you can download:

  • Biotherapeutic Development: Thermo Scientific Solutions for Intact-Protein Analysis (brochure)
  • Monoclonal Antibody Characterization: Achieving Higher Throughput and Productivity (brochure)
  • Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD (application)
  • Reversed-Phase Analysis of a Monoclonal Antibody on an Accucore 150-C4 HPLC Column (application)

Let us know using the Comments box below if these were helpful to you in your work. Also, do submit any questions you might have and our experts will be pleased to respond to you. We look forward to hearing from you.