After writing about many High Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD) applications on this blog, I came across this technical note on how to configure your high-pressure ion chromatography system with electrochemical detection for HPAE-PAD analysis, and thought it would make an interesting read. As many of you working in this area of analysis know this type of analysis is best known for the analysis of carbohydrates (including mono-, di-, tri-, oligo- and polysaccharides), sugar alcohols, and amino sugars.
Electrochemical detection (link above) is used in combination with ion chromatography to detect the presence of compounds that contain an oxidizable or reducible moiety within their structure, and, because this detection method is very sensitive (femtomole detection capability) and requires no derivitization, it saves time and money.
Technical Note 136: Configuring a High-Pressure Dedicated Capillary IC System for Electrochemical Detection discusses how to configure and install our high-pressure, capillary Ion Chromatography system (Thermo Scientific Dionex ICS-4000 HPIC system; link in first para above) for HPAE-PAD analysis using an electrochemical cell with disposable working electrodes. The technical note includes details on the equipment you will need; how to configure the system and components using our Chromatography Data System (CDS) software (Thermo Scientific Dionex Chromeleon CDS software); plumbing the autosampler; installing the electrochemical cell with the electrode; and, the conditioning procedures.
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A little bit about our disposable electrodes: Each lot of electrodes is tested for key application performance and is also serialized for tracking purposes. Typically, each disposable electrode is guaranteed to last for two weeks of continuous use under typical operating conditions (one week for amino acid applications).
Here are two recent blog posts on HPAE-PAD applications that you might be interested in reviewing:
Do let us know if you have questions on the system setup using the Comments box below. Our application chemists will be pleased to hear from you.