I am pleased to share a recently released application note plus a recent webinar, now available on-demand, for the gas chromatography-mass spectrometry (GC-MS) analysis of pesticides in food.
First, the method, which was developed in collaboration by chemists from our Mumbai (India) and Singapore offices along with researchers from the National Research Center for Grapes (India). This reliable and routine method determines dithiocarbamate fungicides (DTCs) with high precision in vegetable and fruits, and is described in Application Note 10333, Analysis of Dithocarbamate Pesticides by GC-MS (downloadable PDF). The fungicides are widely used in agriculture as they they provide a broad spectrum of activity against various plant pathogens, low acute mammal toxicity, and low production costs. But, the DTC moiety is not stable and as it is highly reactive, it chelates with most heavy metals including reacting with sulfhydryl groups of proteins, and becomes neurotoxic, teratogenic, and cytotoxic.
For the method development, the chemists used one of our multi-channel gas chromatographs (Thermo Scientific TRACE GC Ultra gas chromatograph) equipped with a liquid sampler (Thermo Scientific TriPlus RSH liquid autosampler) coupled to one of our ion trap mass spectrometers (Thermo Scientific ITQ 900 ion trap mass spectrometer). The researchers tested the method on two of our HPLC columns, the first a medium-polarity gas chromatography column (Thermo Scientific TraceGOLD TG-624 GC column) and the second, a low-polarity gas chromatography column (Thermo Scientific TraceGOLD TG-5MS GC column) and concluded that either column could be used in the method.
The fresh fruits and vegetables tested included untreated potatoes, tomatoes, eggplants, green chiliies, and grapes, and the control samples came from an organic farm near the city of Pune, India.
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Regulatory Pesticide Residue Analysis in Food: An On-Demand Webinar
In this webinar, titled, Validation of a Routine Multi-Residue Pesticide Method Using QuEChERS and GC-MS/MS Workflow Solutions, (link to registration page after which the webinar begins to play), our experts discuss the stringent European Regulation 396/2005 (downloadable PDF) and Commission Directive 2006/125/EC (downloadable PDF) and present sample preparation and workflow solutions.
Specifically, they discuss how to implement and validate an advanced multi-residue pesticide method in a variety of food matrices including sample preparation using the QuEChERS method and a complete workflow solution featuring triple quadrupole GC-MS technology designed specifically for this type of analysis.
We have a large library of chromatography applications for food and beverage safety on this blog that you might be interested in viewing.
There is a brief Q&A session including in the recording but if your questions are not answered in the webinar, do enter them in the Comments box below; our experts will be pleased to respond to your questions.