Did you know that the leaders in production of biofuels are the United States, France, Sweden, and Germany? And, the goal in the U.S. is to replace 75% of its imported oil by 2025 by alternative power sources including biofuels? (Source & Link Wikipedia).
The application note presented in this post presents two robust ion chromatography HPAE-PAD methods to identify uronic acids in acid-hydrolyzed wood samples, which are a preferred source for biofuel and biochemical production as they are available year round and are not part of the food supply. In addition, accurate determination of both mono- and disaccharides and the uronic acids in biomass materials is important because compositional analysis enables evaluation of conversion yields as carbohydrate content is directly proportional to bioalcohol yield. Therefore, these new methods of analysis will be extremely useful to help biofuel and biochemical production plants maximize their yield.
Application Note 1091, Determination of Uronic Acids and Wood Sugars in Wood-Based Hydrolysate, (downloadable PDF), describes the following two methods:
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- Method 1 is a single high-performance anion-exchange chromatography with pulsed amperometric detection method that uses a mono- and disaccharide column for wood sugars and uronic acids (Thermo Scientific Dionex CarboPac PA20 column). This method has a long run time and multiple dilutions of samples must be analyzed to quantify all the analytes. All 12 analytes of interest (10 wood sugars and 2 uronic acids were separated in less than 40 mins.
- Method 2 is faster and uses an oligosaccharide column (Thermo Scientific Dionex CarboPac PA200 column) to determine the uronic acids in the acid-hydrolyzed wood samples in less than 18 mins. For details on this method, do check out the following application notes:
- Application Note 282, Rapid and Sensitive Determination of Biofuel Sugars by Ion Chromatography, (downloadable PDF).
- Application Update 192, Carbohydrate Determination of Biofuel Samples (downloadable PDF).
One of our our lastest high-pressure ion chromatography systems (Thermo Scientific Dionex ICS-5000+ HPIC system) was used in the development of both methods.
- On-demand, 60-min webinar, Feedstock characterization and fermentation monitoring of biofuels.
- A small and growing library of chromatography biofuel applications on this blog.
- You might also be interested in our Chromatography Solution Online Center which features many useful and complimentary chromatography tools which can help speed up your analysis. The site is updated on a monthly basis; therefore, do check out the Archives section to see what was previously featured.
If you have questions on the method, column, or instrument, do enter them in the Comments box below; our experts look forward to hearing from you.