analysis of Uronic Acids in BiomassDid you know that the leaders in production of biofuels are the United States, France, Sweden, and Germany? And, the goal in the U.S. is to replace 75% of its imported oil by 2025 by alternative power sources including biofuels? (Source & Link Wikipedia).

The application note presented in this post presents two robust ion chromatography HPAE-PAD methods to identify uronic acids in acid-hydrolyzed wood samples, which are a preferred source for biofuel and biochemical production as they are available year round and are not part of the food supply. In addition, accurate determination of both mono- and disaccharides and the uronic acids in biomass materials is important because compositional analysis enables evaluation of conversion yields as carbohydrate content is directly proportional to bioalcohol yield. Therefore, these new methods of analysis will be extremely useful to help biofuel and biochemical production plants maximize their yield.

Application Note 1091, Determination of Uronic Acids and Wood Sugars in Wood-Based Hydrolysate, (downloadable PDF), describes the following two methods:

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  • Method 1 is a single high-performance anion-exchange chromatography with pulsed amperometric detection method that uses a mono- and disaccharide column for wood sugars and uronic acids (Thermo Scientific Dionex CarboPac PA20 column). This method has a long run time and multiple dilutions of samples must be analyzed to quantify all the analytes. All 12 analytes of interest (10 wood sugars and 2 uronic acids were separated in less than 40 mins.

One of our our lastest high-pressure ion chromatography systems (Thermo Scientific Dionex ICS-5000+ HPIC system) was used in the development of both methods.

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