muconic acid HPLC analysisRecently, I tweeted this really heartwarming and environment benefiting story on the development of an ethanol stove for cooking food in Mozambique that could save old-growth forests (link to The New York Times). But, as I did a little research, I found some very sobering statistics hidden behind this story, namely almost half the world’s population cooks on indoor wood fires and leaky stoves leading to more than a million deaths a year due to respiratory diseases. Even more tragic is the fact nearly 50% of the pneumonia deaths in children under five years of age is due to this indoor smoke. (Statistics and link to WHO site.)

After our very successful biofuels webinar (now available on-demand), I have been keeping a close eye on studies on biofuels and here’s a recent one that crossed my desk.

The study, titled, Biosynthesis of cis,cis-Muconic Acid and Its Aromatic Precursors, Catechol and Protocatechuic Acid, from Renewable Feedstocks by Saccharomyces cerevisiae, uses one of our HPLC systems for the analysis. Note that only the abstract is free.

Another interesting use of HPLC systems for biofuels research is increasingly in the use of such systems for lipid profiling which is commonly done using gas chromatography (GC). The advantage of HPLC analysis is that you can eliminate the chemical derivatization step necessary for GC analysis of lipids. Check out more details on our webpage on HPLC lipid profiling for biofuels.

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The poster, Novel HPLC-Based Approach for the Global Measurement of Lipids, downloadable PDF) describes “several HPLC methods for the determination of different lipid classes, including a universal, reversed-phase (RP) method that can resolve steroids, free fatty acids, free fatty alcohols, phytosterols, monoglycerides, diglycerides, triglycerides, phospholipids, and paraffins in a single run.”

Our charged aerosol detector (Thermo Scientific Dionex Corona ultra Detector) was used in the experiments to quantify the lipids to low-level amounts, typically

Lastly, do check out other chromatography applications for biofuels on this blog.

Let us know if you are looking for a specific biofuels application in the Comments box below: our application chemists will be pleased to hear from you.