Our recent issue of the BioPharma enewsletter featured a recently released high-resolution HPLC glycan column (Thermo Scientific GlycanPac AXH-1 column) developed for the separation of charged glycans that coelute from conventional amide HILIC columns. This new column can be used for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules, such as proteins, by fluorescence detection (Thermo Scientific Dionex UltiMate FLD-3400RS Rapid Separation Fluorescence Detector) or mass spectrometry (MS) detection (Thermo Scientific Q Exactive Benchtop LC-MS/MS) methods.
Note that for both detection methods, the researchers used our recently released bio UHPLC system (Thermo Scientific Dionex UltiMate 3000 BioRS system) intended for analysis of proteins, peptides, and biotherapeutics.
Examples presented in the poster note, titled, Novel Glycan Column Technology for the LC-MS Analysis of Labeled and Native N-Glycans Released from Proteins and Antibodies, (link to downloadable PDF) include:
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- Separation of 2AB labeled N-glycans were separated from bovine fetuin by charge, size, and polarity followed by the comparison of the same glycan standards and 2AB-N-glycans. Both fluorescence detection and MS detection methods were explored. This was followed by a comparison using a commercially available amide HILIC column using MS detection.
- LC-MS separation and analysis of native glycans from MAbs and other proteins.
- LC-MS structural analysis of N-glycans from antibodies.
- Charge-based quantitative determination of glycans.
For more technical details on the column including ordering information, check out the glycan column datasheet (link to downloadable PDF).
Do you have questions on the new column? If so, do let us know via the Comments box below. We look forward to hearing from you!