sample preparation HPLC Last month, I featured our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates) designed specifically for sample preparation for bioanalysis experiments in a blog post, titled, Solid-Phase Extraction (SPE) Micro Elution Plates for Bioanalysis and, here, I am pleased to present a recently application note featuring the use of these well plates.

As mentioned in the earlier post, bioanalysis is moving toward methods that require higher levels of sensitivity and reproducibility with decreasing sample volumes, and Application Note 20941, SOLAμ for pre-analysis sample concentration, (downloadable PDF), demonstrates how the use of these new well plates enhances sample pre-concentration by 20X before analysis. This provides the benefits of greater limits of sensitivity, high level of analyte recovery, and accuracy and precision in analysis. In addition, as there is no need for frits, it results in highly consistent results at low elution volumes. Additional benefits include reduced workflow and stability for analytes susceptible to loss or degradation during evaporation and reconstitution.

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The workflow necessary to achieve the 20X increase in sample concentration is included in the application note and method development was done using one of our rapid-separation HPLC systems (Thermo Scientific Dionex UltiMate 3000 RSLC system) equipped with one of our reversed-phase LC-MS columns (Thermo Scientific Accucore RP-MS HPLC column) and a triple stage quadruple mass spectrometer (Thermo Scientific TSQ Vantage triple stage quadruple mass spectrometer).

Additional Resources

  • Our recently released Chromatography Solution Online Center features many useful and complimentary chromatography tools which can help speed up your analysis. The site is updated on a monthly basis; therefore, do check out the Archives section to see what was previously featured.

Let us know if you have any questions on these new plates in the Comments box below. We look forward to hearing from you.