microcystins analysisThis recent story on the high prevalence of microcystin (toxin produced by cyanobacteria) in freshwater lakes in Canadian lakes, had me searching our application database on analytical solutions for microcystin. Sure enough, I found an application that I am very pleased to discuss in this blog post.

By the way, microcystin (Wikipedia page) is highly toxic to plants and humans, can cause serious liver damage in humans, and is considered as a risk factor for cancer. The linkage between the high prevalence of cyanobacteria in the Canadian lakes is the nutrient pollution in these lakes, and scientists are calling for additional studies on this phenomenon plus on how to improve water quality in such lakes. The World Health Organization (WHO) has proposed a provisional guideline concentration of 1.0 μg/L for microcystin-LR in drinking water. (Cyanobacterial toxins: Microcystin-LR in Drinking-water [downloadable PDF])

Poster Note 70078, Sensitive Determination of Microcystins in Drinking and Environmental Waters, (downloadable PDF) describes a simple, fast, and effective on-line SPE method followed by UV detection on a dual HPLC system (Thermo Scientific Dionex UltiMate 3000 ×2 Dual HPLC system). This dual HPLC technology makes it easy to load an untreated sample onto an extraction column (using one pump) and then to wash the analytes from this column onto an analytical column using a second pump.

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Rapid separation HPLC columns (Thermo ScientificAcclaim PA2) were used for the on-line SPE step and for analysis. The on-line SPE wizard in the Chromatography Data System (CDS) software (Thermo Scientific Dionex Chromeleon CDS) was used to develop a fully automation method with strict process control.

This application note was developed at our Shanghai Applications Lab.

Let us know if this application was helpful to you: do enter any questions you might have in the Comments box below. We look forward to your comments.