chromatography plant physiology studiesIt has been a few months since I featured any chromatography methods in botany research studies, and here, I am pleased to present several peer-reviewed articles feature the use of our ion chromatography systems and columns.

Study: Is white clover able to switch to atmospheric sulphur sources when sulphate availability decreases?

In this fascinating study, researchers from INRA/Université de Caen (France) developed a novel method to measure sulfur (an essential element for plant growth) acquisition by Trifolium repens L., white clover, pictured in this post. Clover is a major legume plant in the Western European grasslands and, research is showing that the sulfur is decreasing in soils globally. The purpose of the study was to test if clover plants can increase their ability to use atmospheric sulfur when sulphate availability decreases in the soil.

The results indicate that clover is able to enhance its ability to absorb atmospheric sulfur when sulfur in the soil decreases. Sulphate analysis was done using of one of our earlier ion chromatography systems (Thermo Scientific Dionex DX 100 Ion Chromatograph).

Study: Organ-coordinated response of early post-germination mahogany seedlings to drought

In this first study, (click the title above to access the abstract; only the abstract is free), researchers from two Brazilian institutions (Universidade Federal de Minas Gerais and Instituto de Botânica), “studied the effect of water deficit on morphological and biochemical responses in different organs of newly germinated mahogany (Swietenia macrophyllaKing) seedlings, a woody species that occurs in the Amazon rainforest.” By the way, did you know that logging of old-growth mahogany is one of the prime causes of deforestation in the rainforests of Brazil, and that 80-90% of Peruvian mahogany imported to the United States is illegally harvested? (Source & link Wikipedia).

Using one of our integrated ion chromatography systems (Thermo Scientific Dionex ICS-3000 ion chromatography system) and one of our carbohydrate ion chromatography columns (Thermo Scientific Dionex CarboPac PA1 Analytical column), the researchers used the HPAE-PAD technique for the analysis of fructose, glucose, sucrose and myo-inositol levels in leaves and stems during drought conditions.

In their surprising results they found that mahogany seedlings reacted to water deficits “by triggering biochemical processes that resulted in the attenuation of oxidative stress and the establishment of osmotic adjustment,” and that the seedlings could germinate in conditions where water was severely limited.

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Study: Initial Plant Growth in Sand Mine Spoil Amended with Peat Moss and Fertilizer Under Greenhouse Conditions: Potential Species for Use in Reclamation

In this study, (click the title above to access the full, free article), researchers looked at the use of two legume species, sundial lupine (Lupinus perennis) and Illinois bundleflower (Desmanthus illinoensis), and two warm-season grass species, Indian grass (Sorghastrum nutans) and little bluestem (Schizachyrium scoparium) as species to be used in reclamation of sand dunes that been mined for sand and, hence, lost significant soil quality. This study was conducted in the sand dunes of the Great Lakes Basin are of the USA which is the largest collection of freshwater sand dunes in the world.

As per their results, the researchers recommend that “sundial lupine is recognized as a primary candidate for sand mine reclamation, while Illinois bundleflower is also recommended as an appropriate species for revegetation efforts. We recommend using soil amendments that are functionally equivalent to peat in increasing soil water holding capacity. We further suggest that fertilization may be accomplished by including legumes in plant species mixes used for revegetation. Results presented here may help to identify appropriate species and soil amendments for the reclamation of former sand mines or restoration of freshwater sand dunes.”

Nitrate in the soil was analyzed using of one of our earlier ion chromatography systems (Thermo Scientific Dionex DX 500 Ion Chromatograph).

Study: Genotypic differences in Al resistance and the role of cell-wall pectin in Al exclusion from the root apex in Fagopyrum tataricum

In this study, (click the title above to access the abstract; only the abstract is free), researchers studied aluminum toxicity as it is one of the factors limiting crop production in acid soils. They examined the genotypic differences among eight types of tatary buckwheat (Fagopyrum tataricum) to identify possible mechanisms of aluminum resistance.

The root organic acids were analyzed using one of our integrated ion chromatography systems (Thermo Scientific Dionex ICS-3000 system). As per their results, the researchers concluded: “Although we were unable to establish a significant correlation between Al exclusion and pectin content among the eight cultivars, a strong correlation could be established among six cultivars, in which the pectin content in the most Al-resistant cultivar ‘Chuan’ was significantly lower than that in the most Al-sensitive cultivar ‘Liuku2’.”

Additional Resources

  • You might also be interested in our recently released Chromatography Solution Online Center which features many useful and complimentary chromatography tools which can help speed up your analysis. The site is updated on a monthly basis; therefore, do check out the Archives section to see what was previously featured.

Have you read similar research studies using our ion chromatography systems? If yes, do share in the Comments box below.