liquid chromatography analysis of hormones in waterDid you know that low doses ofpharmaceuticals, such as antibiotics, anticonvulsants, mood stabilizers, and sex hormones among others have been foundincreasingly in environmental and drinking waters? As per the Utah State University site, pharmaceuticals have been found in at least 41 million of America’s drinking water supplies. Last year, due to increasing concerns over the long-term health impacts to humans and aquatic species, the World Health Organization published a 52-page report titled, Pharmaceuticals in drinking water (downloadable PDF).

In 2010, the U.S. EPA published the U.S. EPA Method 539 for determination of hormones in drinking water (link to method PDF) defining the application protocol to use Solid-Phase Extraction (SPE) and Liquid Chromatography, Electrospray Ionization, Tandem Mass Spectrometry (LC-ESI-MS/MS) for the analysis.

To address the challenges of detecting hormones in drinking water, we present two tools for your benefit:

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  • Application Protocol for EPA Method 539 (on-demand webinar; link to registration page from where you can view the webinar) featuring one of our experts on the application protocol plus a kit that will drastically reduce your method development time for U.S. EPA Method 539.
  • Hormone Application Kit (P/NTF-MKIT0011S) designed to significantly reduce your method development time for the method. By following the application protocol and using the products included in the kit, the sensitivity, extraction recovery, and reproducibility of this method can meet the requirements of U.S. EPA 539 and other international regulatory requirements.

The kit includes SPE disks, HPLC column, and vials along with verified instrument methods, detailed sample preparation and analysis protocol, and software method files and application protocol and is intended to be used with the following of our systems: automated SPE instrument (Thermo Scientific Dionex AutoTrace 280 SPE instrument), HPLC system (Thermo Scientific Dionex UltiMate 3000 RSLC system),triple stage quadrupole mass spectrometer (Thermo Scientific TSQ Vantage Triple Stage Quadrupole Mass Spectrometer) and our quantitation and targeted screen chromatography software (Thermo Scientific TraceFinder Software).

The next post on this topic will feature some of the more important questions asked at the end of the webinar especially on the Hormone Application Kit.

Do you have questions after viewing the kit details and the webinar? If so, do let us know via the Comments box below. We look forward to hearing from you!