One of my favourite songs of all time is Me and Mrs. Jones, by Billie Paul. It is quite simply magnificent! Nerdy as I am, it resonates in my head as “me and Mr. Gauss”, especially when I am in a spot of bother and the peaks are not looking perfect. For all you customers out there: do not worry! I do try very hard not to burst out in song when I am visiting you and there are awful-looking peaks.
Yes, I admit I have a crush on Gauss. Watching perfect peaks as a chromatography addict is quite simply thrilling, and it never bores me. After injecting samples on a GC for over 25 years now, it is still a source of happiness to see nice peak shapes. The opposite is also quite true: for all chromatographers it is somewhat saddening to see tailing or fronting peaks.
So what do you do when seeing those not-so-perfect peaks? Did you know we have an excellent troubleshooting guide that will help you in almost all chromatography-related cases?
And where do we find Gauss in the chromatography lab?
Be Gaussian: Chromatography Troubleshooting
Our company has gathered expert knowledge on troubleshooting chromatographic issues. We have complete guides on troubleshooting GC, LC and sample preparation. These are step-by-step guides, but we also have presentations and technical guides available for you, as well as a complete team that is ready to support you with issues and give you recommendations on separations or other typical chromatographic queries.
Like what you are learning?
Be Gaussian with Charlie Chromeleon
Chromeleon CDS is a software that makes it easy to review and report chromatography data, and all of the views and layouts are completely flexible. The client server capability makes it easy to share data and results inside the lab, but also with your peers and colleagues all over the globe.
Another field where Gauss is present is in the lab, and that is when we are performing quality controls. A typical method is to track a sample with a known concentration for one or several analytes and track the deviation. Laboratories are using Gauss to calculate the distributions, and are in control if the concentration falls in the 95% probability range of the bell curve.
Chromeleon CDS software is perfectly capable of tracking quality control results over multiple batches and systems. It can also perform system suitability tests to monitor your chromatographic peaks (link to application note PDF).
Gauss is everywhere in the lab, including in how ions are distributed after being separated in the mass spectrometer and how particles are distributed in an LC column.
Please send me your normal distributions and make my love story with the bell curve exceptional.