Metabolisms and metabolomics have always fascinated me. What compounds do an organism or cell produce and are they indicative of the organism itself, disease, or possible disease prevention? As I sit here this morning drinking coffee I wonder what truly awesome metabolic process created caffeine. As I have read literature on metabolomics I see a lot of work done using LC-MS, among many others. There is some really great work and in many instances that technique is the only one to use for this type of work. However, there are those compounds that are just better analyzed by another complimentary technique, GC-MS. For those of you that really know, the chromatography of this type of sample can be messy at best. There are many times when you just would not be able to separate compounds from one another. That’s where MS/MS comes in. Using SRM, also known as MRM, you can look at one specific compound without the interference of others that may coelute.
The physio-chemical diversity of biological small molecules makes a metabolomics analysis very difficult. Therefore, different analytical strategies are necessary and are ideally combined with each other. Analyses focusing on a group of metabolites as well as watching as many metabolites as possible at specified environmental or developmental stages are called metabolite profiling. On the other hand metabolite target analyses are restricted to specific metabolites of interest, which can be selectively monitored and quantified. However, there is a discrepancy of detectable peaks in a typical metabolomics sample and the number of assignable chemical identifications due to a restricted availability of non-complete reference libraries and technical problems such as ultra complex coelution of compounds and non-optimal mass spectral deconvolution in GC-MS. Therefore, in metabolomics we propose a workflow to increase the number of identifiable and selectively quantifiable chemical structures. The workflow proposal is fully described in the application note.
The TSQ Quantum GC-MS/MS Series provides the discovery phase analysis and very accurate and selective identification and quantitation over a high dynamic range of interesting metabolite markers from complex mixtures using MRM mode. Compounds, which cannot be separated through GC can be separated using compounds specific MRM transitions in one run. This instrument is optimal for use in metabolomics to perform the cycle of metabolite screening on the one hand, and targeted metabolite analysis of complex samples on the other. By this approach, metabolome coverage will increase continuously while accurate and selective identification and quantitation is performed in a high sample throughput manner.
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Oh, one more thing. If there is someone that can describe for me the metabolomic process or processesthat create caffeine I will buy them a cup of coffee. Nothing alcoholic in it, that is a whole different metabolic process. To collect the coffee you will need to find me, I travel around the world and there are several seminar tours and conferences coming up everywhere. When you arrive just ask for me.
Download the application note here, Metabolomics Application