proteomics applicationsThis blog post completes our series on the 14 articles on proteomics research published in a 30-day span in Nature magazine. If this is the first blog you have read in the series, and are wondering why the focus on articles published in Nature magazine when there are so many other distinguished publications featuring articles on this subject, here is why.

Having an article published inNature is not only very prestigious but such articles are often highly cited leading to grant funding and attention from the mainstream media. Also, many of the most significant scientific breakthroughs in modern history have been first published inNature. While most journals tend to be specialized, Nature tends to span across different fields, so the criteria for acceptance is tougher. It is indeed the number one ranked journal in science. Research scientists are the primary audience for the journal, but summaries and accompanying articles are intended to make many of the most important papers understandable to scientists in other fields and the public.

Here are the last five articles in this series.

Article Title:Global profiling of dynamic protein palmitoylation

Synopsis: This article describes how by combining metabolic incorporation of 17-ODYA and SILAC labeling and MS, the authors were able to accurate identify and quantify specifically enriched palmitoylated proteins. Using this approach, they identified and quantified more than 400 palmitoylated proteins in mouse T-cell hybridoma cells. By using 17-ODYA metabolic pulse-chase labeling, they were able to distinguish palmitoylated proteins that undergo rapid turnover from those that are stably modified. Finally, using a lipase-specific inhibitor, they identified a specific set of enzymatically regulated palmitoylated proteins. These findings point to a special population of palmitoylated proteins that, through dynamic regulation by hydrolytic enzymes, are distinguished from bulk protein palmitoylation events.

Article Title: S-nitrosylation of NADPH oxidase regulates cell death in plant immunity

Synopsis: This article describes determining whether NADPH oxidase might be S-nitrosylated using MS analysis.

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Article Title: Molecular mechanism of anaerobic ammonium oxidation

Synopsis: The authors discuss the resolution of genes and proteins central to anammox metabolism and purification of key enzymes that catalyse N2H4 synthesis and its oxidation to N2. They describe resolving the anammox pathway and its enzymes by a combination of complementary approaches with proteomics playing instrumental role.

Article Title: A heteromeric Texas coral snake toxin targets acid-sensing ion channels to produce pain

Synopsis: This article describes the identification of snake venoms capable of activating somatosensory neurons.

Article Title: Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon

Synopsis: This article describes finding the structure, the proposed mechanism and evolution of a CS2 hydrolase from Acidianus A1-3.

Do tell us if you have read any other interesting articles on this topic in the comments field below.