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Easier Peptide Mapping by LC-MS

Peptide mapping is one of several routine methods to characterize biopharmaceutical proteins. For research environments, this technique, if combined with mass spectrometry (MS), is utilized for the characterization and confirmation of the primary sequence of monoclonal antibodies. In addition, peptide mapping can help to identify, localize, and quantitate post-translational modifications (PTMs). Peptide mapping methods are often developed and evaluated with combined UV and MS detection, to simplify the transfer to routine environments where UV detection is used alone. In high-throughput workflows, peptide mapping experiments are performed for antibody identity confirmation, PTM characterization, and stability studies. The new Vanquish Flex UHPLC system features a quaternary pump 1 for highest application flexibility and fully biocompatible flow path. In addition, similar to the Thermo Scientific Vanquish UHPLC system 2 , the sample is pressurized prior to the injection into the high pressure flow path. This results in a highly stable flow delivery and thus significantly improved retention time precision, increasing the confidence in peak assignment in peptide mapping experiments with UV detection. 3

Technique: LC-MS Resource Type: Application Note

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