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Optimizing and Monitoring Solvent Quality for UV-vis Absorption, Fluorescence and Charged Aerosol Detectors1
2014-03-24_19-10-18

Optimizing and Monitoring Solvent Quality for UV-vis Absorption, Fluorescence and Charged Aerosol Detectors

Solvent quality has a large impact on laboratories using liquid chromatography (LC) methods. A minimum purity and filter grade is required to reliably operate an LC system. Low particle content is beneficial for the lifetime of LC pump check valves, pistons, and piston seals. The column depends even more on the absence of particles. Bottled high performance liquid chromatography (HPLC) solvents are typically filtered at least to the 0.22 µm level to remove bacterial contaminants in addition to any particulate matter. This is usuallly sufficient for conventional columns packed with 3 µm or 5 µm particles. When adding buffer salts to the mobile phase, further preparation may

Technique: Liquid Chromatography Resource Type: Technical Note
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