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Tune Your Mixing Volume for Gradient Generation to Achieve Better Detection Limits1
Tune Your Mixing Volume for Gradient Generation to Achieve Better Detection Limits

Tune Your Mixing Volume for Gradient Generation to Achieve Better Detection Limits

This Technical Note discusses two technical approaches for gradient formation and examines the reasons for fluctuations in solvent composition. In case of different extinction coefficients of the used solvents these fluctuations become visible in UV detection resulting in characteristic baseline ripples. At the end we will provide assistance in choosing the right mixing system for best measurement results.

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