Here is the third application note in this series on improving sample preparation for bioanalytical and clinical research applications using our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates). The previous two application notes are presented in the following blog posts:
Application Note 20943, Improving sensitivity with SOLAμ for analytes susceptible to issues during pre-concentration dry down, (downloadable PDF) describes how to pre-concentrate the extract directly on the well plate even with low sample volumes thus removing the need for evaporation steps and, therefore, the associated problems.
For the method development, our chemists used samples of ibuprofen, ibuprofen d3, ketoprofen, and ketoprofen d3, the aforementioned SPE well plates, one of our rapid-separation HPLC systems (Thermo Scientific Dionex UltiMate 3000 RSLC system),one of our reverse-phase MS HPLC columns (Thermo Scientific Accucore RP-MS HPLC column), and a guard cartridge (Thermo Scientific Accucore RP-MS Defender guard cartridge), and one of our mass spectrometers for bioanalysis applications (Thermo Scientific TSQ Vantage mass spectrometer) for the MS/MS detection.
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The method resulted in a fourfold pre-concentration without the need for dry down and even with a low elution volume, excellent results were achieved for both ibuprofen and ketoprofen. In addition, analyte recovery was shown to be greater than 90% for ibuprofen and ketoprofen by comparison to post extraction fortified blank samples.
- Do check out our growing library of bioanalysis applications on this blog.
- Our recently released Chromatography Solution Online Center features many useful and complimentary chromatography tools which can help speed up your analysis. The site is updated on a monthly basis; therefore, do check out the Archives section to see what was previously featured.
Let us know if you have any questions on these new plates in the Comments box below. We look forward to hearing from you.