Jeannie Lee, Ph.D and Wilhelm Haas, Ph.D., from Massachusetts General Hospital and Harvard Medical School discuss their collaboration characterizing the Xist interactome, a long noncoding RNA that oversees the silencing of one of the two X chromosomes—the inactive X (Xi)—in female mammals. The team developed an RNA-centric proteomic method called identification of direct RNA-interacting proteins (iDRiP). One component of iDRiP is TMT-based multiplexed quantitative analysis using a Thermo Scientific Orbitrap Fusion mass spectrometer.
A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.
Minajigi A, Froberg JE, Wei C, et al.
High Throughput Quantitative Proteomics Using Isobaric Tags